Description：

Methotrexate (MTX), analog of folic acid, is a nonspecific inhibitor of the dihydrofolate reductase(DHFR) of bacteria and cancerous cells as well as normal cells. It forms an inactive ternary complex with DHFR and NADPH.

Chemical Information：

Molecular Weight (MW)		454.44
Formula		C20H22N8O5
CAS No.		59-05-2
Solubility (25°C) *	DMSO	90 mg/mL warmed (198.04 mM)
	Water	Insoluble
	Alcohol	Insoluble

Preparing Stock Solutions

Concentration Volume (DMSO) Mass	1 mg	5 mg	10 mg
1 mM	2.2005 mL	11.0026 mL	22.0051 mL
5 mM	0.4401 mL	2.2005 mL	4.4010 mL
10 mM	0.2201 mL	1.1003 mL	2.2005 mL
50 mM	0.0440 mL	0.2201 mL	0.4401 mL

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